Antibodies for immunocytochemistry
Item
Supplier
Catalog no. Alternative
Nestin (Ms IgG1) ßIII-tubulin (Rb polyclonal) MAP2 (Ms IgG1) NeuN (Ms IgG1,
clone A60) Neurofilament 200
(Ms IgG1) GFAP (Ms IgG1) S-100ß (Ms IgG1) Myelin proteolipid protein (Ms IgG2a)
Becton-Dickenson N17220-050 Covance Research PRB-435P Products, Inc.
Sigma Chemicon
Sigma
Sigma Sigma Chemicon
M4403 MAB377
N-0142
G-3893 S 2532 MAB388
Chemicon AB5922 (Rb) Cymbus CBL412
(Ms IgG1) Chemicon AB5622 (Rb) Abcamab13938
(Ms IgG1, clone 4G2) Chemicon AB1981 (Rb)
Santa Cruz sc-7170 (Goat) Swant 37 (Rb) Calbiochem NB38 (Ms IgG2a, clone plpc 1) (Continued)
|
Myelin basic protein |
Chemicon |
MAB386 |
Chemicon AB5864 (Rb) |
|
(Rt IgG2a) | |||
|
O4 (Ms IgM, |
Chemicon |
MAB345 |
Sigma O7139 (Ms IgM, |
|
clone 81) |
clone O4) | ||
|
PDGFR-a (Rb IgG) |
Santa Cruz |
sc-338 |
Abcam ab20274 |
|
(Ms, clone SPM473) | |||
|
FITC-donkey a-mouse |
Jackson |
715-095-150 |
Molecular Probes |
|
Laboratories |
A-21042 (goat | ||
|
a-Ms, Alexa 488) | |||
|
Cy3-donkey a-mouse |
Jackson |
715-165-150 |
Molecular Probes |
|
Laboratories |
A-21426 (goat a-Ms, | ||
|
Alexa 555) | |||
|
Cy5-donkey a-mouse |
Jackson |
715-175-150 |
Molecular Probes |
|
Laboratories |
A-21238 (goat a-Ms, | ||
|
Alexa 647) | |||
|
FITC-donkey a-rabbit |
Jackson |
711-095-152 |
Molecular Probes |
|
Laboratories |
A-21206 (donkey | ||
|
a-Rb, Alexa 488) | |||
|
Cy3-donkey a-rabbit |
Jackson |
711-165-152 |
Molecular Probes |
|
Laboratories |
A-31572 (donkey a-Rb, | ||
|
Alexa 555) | |||
|
Cy5-donkey a-rabbit |
Jackson |
711-175-152 |
Molecular Probes |
|
Laboratories |
A-31573 (donkey a-Rb, | ||
|
Alexa 647) | |||
|
FITC-donkey a-rat |
Jackson |
712-095-153 |
Molecular Probes |
|
Laboratories |
A-21042 (goat a-Rt, | ||
|
Alexa 488) | |||
|
Cy3-donkey a-rat |
Jackson |
712-165-153 |
Molecular Probes |
|
Laboratories |
A-21426 (goat a-Rt, | ||
|
Alexa 555) | |||
|
Cy5-donkey a-rat |
Jackson |
712-175-153 |
Molecular Probes |
|
Laboratories |
A-21238 (goat a-Rt, | ||
|
Alexa 647) | |||
|
Normal donkey serum |
Jackson |
017-000-121 |
Normal goat serum |
|
Laboratories |
(005-000-121) | ||
|
Reagents | |||
|
Item |
Supplier |
Catalog no. |
Alternative |
|
Neurobasal medium |
Gibco |
21103-049 |
D-MEM/F12 medium |
|
(Gibco) | |||
|
B27 supplement |
Gibco |
17504-044 |
N2 supplement (Gibco |
|
17502-048) | |||
|
Penicillin/streptamycin |
Gibco |
15140-122 |
Omega PS-20 |
|
Gentamicin |
Sigma-Aldrich |
G-1397 |
Gibco 15710-064 |
|
Fungizone |
Omega |
FG-70 | |
|
FGF2 |
Invitrogen |
13256-029 |
Sigma F-0291 |
|
Hoechst 33342 |
Invitrogen |
H1399 |
DAPI (Invitrogen D1306) |
|
(Continued) | |||
|
Item |
Supplier |
Catalog no. |
Alternative |
|
Qtracker 525 cell |
Invitrogen |
Q25041MP |
Other kits have Qdots |
|
labeling kit |
with Em of 565-800 nm | ||
|
Accutase or Accumax |
Innovative Cell |
AT-104 |
Trypsin 0.05% EDTA |
|
Technologies, Inc. |
AM-105 |
(Gibco 25300-062) | |
|
D-AP5 |
Tocris |
0106 |
Calbiochem 165304 |
|
Bioscience | |||
|
IsoFlo (isoflurane) |
Abbott |
05260-05 |
Avertin or pentobarbitol |
|
( + )-Bicuculline |
Tocris |
0130 |
Sigma B-9130 |
|
Bioscience | |||
|
Biocytin |
Sigma-Aldrich |
B-4261 | |
|
Calcium chloride |
Sigma-Aldrich |
C-5080 |
Calbiochem 208291 |
|
Cesium chloride |
Sigma-Aldrich |
C-3032 |
Gibco BRL 15507-015 |
|
Cesium hydroxide |
Sigma-Aldrich |
C-8518 | |
|
CNQX, disodium salt |
Tocris |
1045 |
AG Scientific C1053 |
|
Bioscience | |||
|
Y-Amino-«-butyric |
Tocris |
0344 |
Sigma A-5835 |
|
acid (GABA) |
Bioscience | ||
|
Glucose |
Sigma-Aldrich |
25,307 |
Calbiochem 346351 |
|
L-Glutamic acid |
Tocris |
0218 |
Calbiochem 3510 |
|
Bioscience | |||
|
Glycine |
Tocris |
0219 |
Calbiochem 3570 |
|
Bioscience | |||
|
HEPES |
Sigma-Aldrich |
H-3375 |
Calbiochem 391340 |
|
Magnesium ATP |
Sigma-Aldrich |
A-9187 | |
|
Magnesium chloride |
Sigma-Aldrich |
M-4880 |
Calbiochem 442611 |
|
NMDA |
Tocris |
0114 |
Calbiochem 454575 |
|
Bioscience | |||
|
Phalloidin |
Calbiochem |
516640 | |
|
Picrotoxin |
Tocris |
1128 |
Calbiochem 528105 |
|
Bioscience | |||
|
Potassium chloride |
Sigma-Aldrich |
P-3911 |
Calbiochem |
|
Potassium gluconate |
Sigma-Aldrich |
P-1847 |
Calbiochem 346125 |
|
Potassium phosphate |
Sigma-Aldrich |
P-0662 |
Calbiochem 529568 |
|
(monobasic) | |||
|
Sodium bicarbonate |
Sigma-Aldrich |
S-6297 | |
|
Sodium chloride |
Sigma-Aldrich |
20,443-9 |
Calbiochem 56744 |
|
Sodium hydroxide |
Sigma-Aldrich |
S-8045 |
Calbiochem 567530 |
|
Sodium phosphate |
Sigma-Aldrich |
S-0876 |
Calbiochem 567547 |
|
(dibasic) | |||
|
Sucrose |
Sigma-Aldrich |
S-7903 |
Calbiochem 5737 |
|
Tetraethylamonium |
Sigma-Aldrich |
T-2265 |
Calbiochem 584128 |
|
chloride (TEA-Cl) | |||
|
Tetrodotoxin (TTX) |
Tocris |
1078 |
Calbiochem 554412 |
|
Bioscience | |||
|
Water (HPLC grade) |
Sigma-Aldrich |
27,073-3 |
Fisher W5-4 |
|
Bone wax |
Fine Science |
19009-00 |
Harvard Apparatus |
|
Tools |
(no. 599864) |
Average user rating: 5 stars out of 1 votes
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